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TB-500 has been particularly extensively studied in cardiac repair models with foundational research demonstrating that thymosin beta-4 and TB-500 promote cardiomyocyte survival following ischaemia-reperfusion injury, stimulate cardiac progenitor cell migration into infarcted tissue, reduce infarct size, and improve cardiac function in myocardial infarction models

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At low to moderate concentrations, they stimulate cellular activity

- Fasting glucose and a basic lipid panel (AOD-9604 did not raise glucose or worsen lipids in trials, but baseline confirmation is useful)