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As shown in Section Riboflavin Ameliorates Oxidative Stress, Mitochondrial Dysfunction, and Neuroinflammation, riboflavin has antioxidant properties and upregulates antioxidant enzymes especially SOD, also, supplementation with riboflavin will elevate the intra-mitochondrial FAD levels compensating for the decline in the capacity to assemble of dysfunctional mitochondrial complexes

Thus it is vital to follow a specific process within 30 minutes of collection: Collect blood plasma (using EDTA or heparin to prevent coagulation) and then centrifuge the sample to remove the blood cells from the plasma