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The concentration of serotonin can be modulated by the gut microbiome in multiple ways [230], most notably shown by altered concentration of serotonin and its metabolites in the hippocampus of germ-free animals [231]

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Following primary antibody incubation, cells were treated with goat anti-rabbit IgG H&L Alexa Fluor 488 (ab150077, Abcam), donkey anti-goat IgG H&L Alexa Fluor 647 (ab150131, Abcam) and DAPI (Invitrogen) for 1 hour in the dark at room temperature