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Representative samples were excised on dry ice, added to extraction solution, and homogenised under dry ice vapour using a Precellys24 bead-based homogeniser (Bertin Instruments) for 3 20 s at 5000 rpm

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2.3 DSS-induced models and GHK-Cu treatment DSS is extensively utilized in animal models of UC due to its capacity to induce histopathological, clinical, and immunologic responses that closely resemble the manifestations observed in patients with IBD ( 2.4 Evaluation of colitis Throughout the animal experiment, the mental state, stool consistency, fur quality, perianal condition, and fecal occult blood of the experimental mice were assessed daily at a fixed time