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The membranes were blocked with 5 % skim milk (dilution in TBS with 0.05 % Tween 20 (TBST)) and incubated with primary anti-bodies (1:1000, dilution in TBST) for overnight at 4C

PMCID: PMC4075452 Griffith O, Meister A (1979) Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine)

The ketogenic diet is reported to be a promising approach for social deficits seen in ASD by regulating gene expression and histone acetylation in the brain [49]

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To separate a phenotype caused by Gpx4 -deficiency from that of Cre side effects, tamoxifen-treated mice harboring Gpx4 fl/fl ;Cre ERT2 and Gpx4 wt/wt ;Cre ERT2 BM cells were allowed to recover and blood was drawn every three weeks (Figure 1)