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and 5^* and 2^* is the multiplicity of the dilution of the serum and the protein removing reagents after mixing

The orientation of NADPH within the crystal structure of the SmTGR (U597C)NADPH complex (Figure 2), along with a previously performed kinetic isotope effect with Pro-S NADPD (Smith et al., 2023), establishes that the hydride transferred to the FAD of SmTGR from NADPH is from the Pro-S position and permits assignment of the observed NMR signals

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