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This normalization was validated by actual colony counts to confirm that each sample contained 1 10 8 CFU/mL before RNA extraction

In addition, NFB, a well-known downstream factor of NRF2, is a central activator of the immune response

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Under sterile conditions, rats were deeply anesthetized by intraperitoneal injection of ketamine (90 mg/kg) and xylazine (80 mg/kg) mixture, and were placed in a stereotaxic apparatus, a heating lamp was on during the surgery to keep the animal warm, and ophthalmic ointment was applied to prevent corneal drying

The distinctive SCAN pattern (SCAN detection is described in the Methods), which alternates with foot, hand, mouth motor regions along the central sulcus, was evident in both patients with PD and HC individuals (Fig