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Materials and methods Rat model of soft tissue injury Animal studies and procedures were carried out in accordance with an approved protocol from the Institutional Animal Care and Use Committee (IACUC) at the Fourth Military Medical University (IACUC-20220505)

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For the subsequent validation experiments using reconstituted cell lines, approximately 1 10 6 STK38-deficient cells expressing either RIPK1 WT or the S309A mutant were prepared and injected using the same procedure