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Once the senescence response was established, cells were treated with 100 nM Bafilomycin (Sigma, B1793) for 1 h in the incubator, followed by the addition of 30 M C 12 FDG for 1 h in the incubator

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Dosage regimens are adjusted to provide the optimum desired response (e.g., a therapeutic response)

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In one approach, [ 32 P]-PPi is used for the reaction