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In fact, the content of glucosinolates in cruciferous vegetables can be significantly affected by the storage conditions

10.1007/s13337-020-00598-8 140 MehrbodP.AbdallaM

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Tumors were dissected from individual mice and weighed by two blinded researchers

That means that 11% of people with Hashimotos would have been missed if you were only looking at TPO antibodies, and a full 35% of patients with Hashimotos would have been missed if you were only looking at thyroglobulin antibodies