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High metabolite cultivation To assess the effects of elevated extracellular metabolites, complete DMEM (5% FBS) was supplemented with 20 mM of each indicated metabolite as following: glutamine, glutamate plus NHCl (equimolar), L-aspartate plus L-citrulline (equimolar), L-arginine

This would exclude the possible nonspecific contribution of the additional volume (as it may be the infusion into the anterior chamber to increase intraocular pressure above systolic pressure to induce ischemia ( Finally, the obtained findings should be summarized with the previous eye evidence

Importantly, senescent cells while dead in terms of clonogenicity continue to be viable and metabolically active, over time developing a specific expression pattern of immunomodulatory factors

For fertility stress, nesfatin-1s link to increased anxiety raises red flags

2004 Sep;35(9):2072-7