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Usually this one dose is enough to stop symptoms and treat the problem

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Plays a key role in cellular health, detoxification, immune function and fighting against oxidate stress

32 Zinc acts as a cofactor for enzymes of the antioxidant system, and inhibits the enzyme, nicotinamide adenine dinucleotide phosphate oxidase, a prooxidant enzyme, and induces metallothionein synthesis, which is important in the reduction of hydroxyl radicals

Some signs of B12 deficiency in chickens include: Leg weakness Nervous system impairment Perosis Poor feathering Reduced hatch rates (from deficient parents embryos frequently die at around 17 days old) Most commercial chicken feed contains B vitamins, so B12 deficiency is less common