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ZSGB-Bio, Beijing, China) diluted in TBS (containing 1% BSA) for 1 h, and reacted with ECL Prime Western Blotting Detection Reagent (Beyotime Institute of Biotechnology)

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It facilitates the transport of extracellular cystine into cells, where it is reduced to cysteine, a critical precursor for the synthesis of glutathione (GSH)