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All efforts were made to minimize the number of mice used and decrease animal anguish

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cerevisiae transformed with LacAnc100 or LaccID was used to inoculate 30 ml of SC minimal medium supplemented with raffinose (in a 100-ml flask) and incubated for 48 h at 30 C and 220 rpm (Orbitron shaker, INFORS)

The concept of computational drug design, which is grounded in protein sequence information, has significantly advanced with the introduction of TransformerCPI2.0 by Mingyue Zheng et al., as depicted in Fig

These investigations have suggested that some cancers, such as leukaemia, are driven by metabolic alterations and thus appropriate metabolic-based treatment strategies are needed (32, 33, 154)