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Cell 73 , 354363.e353 (2019)

After the incubation for 30 min at 30 C the reactions were stopped with 0.5 mg/mL proteinase K and 0.1% SDS, and incubated for 5 min at 37 C

After a ten-second wait, the grids were blotted with filter paper and immediately immersed in liquid nitrogen-cooled liquid ethane

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Response timelines depend on severity and chronicity of stress-sleep disruption, underlying causes (acute situational stress versus chronic nervous system dysregulation), genetic factors affecting sleep and stress response systems, concurrent sleep hygiene and stress management practices that amplify benefits, protocol adherence and dosing optimization, and individual neurological responsiveness