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Kornblau, SM, Qiu YH, Womble WF, Cade JS, Jackson CE, Milella M, Konopleva M, Andreeff M

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P.BeaulieuJ

2.7 Preparation of mitochondria and assay of enzyme activity The enzyme activity of three respiratory-chain complexes was quantified in the mitochondria of L-02 hepatocytes treated with CGA and in untreated cells, as previously described ( 2.8 Quantitative analysis of oxidized and reduced forms of glutathione (GSH) To detect GSH and GSSG (glutathione disulfide), two 200 L sample solutions were prepared