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Abbreviations CGRP: Calcitonin gene-related peptide CSD: Cortical spreading depolarization REFORM: Registry for Migraine ICHD-3: International Classification of Headache Disorders, 3rd edition IQR: Interquartile range FHM: Familial hemiplegic migraine TCC: Trigeminocervical complex BOLD: Blood oxygenation level-dependent References Stovner LJ, Nichols E, Steiner TJ (2018) Global, regional, and national burden of migraine and tension-type headache, 19902016: a systematic analysis for the global burden of Disease Study 2016

Xia et al., 2025d) Looking ahead, research should focus on validating tissue-specific biomarkers and integrating spatial metabolomics with artificial intelligence technology to refine treatment strategies ( Statements Author contributions H-HX: Visualization, Writing original draft, Writing review & editing

The Arterial EC3 subgroup, which is enriched in the mesenteric sample and therefore represents ECs from larger arteries, surprisingly shows higher levels of cell cycle genes compared to other arterial subpopulations (Fig

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