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For the test, first a blood sample is taken to measure plasma holoTC, after which a dose of 9g of crystalline B12 in water is given orally at 6h intervals over a 24h period

The distances migrated by individual bands of the standard proteins to the edge of the gel during electrophoresis are plotted against their respective pI values, thereby generating a standard curve

Vitamin D Rogan says he takes around 5000IU of vitamin D daily

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However, the analysis can now be performed on a single blood spot, and the cost has come down to a reasonable amount