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In this study, we combined correlative light and electron microscopy (CLEM), 3D electron tomography (ET), cryo-EM, and all-atom molecular dynamics simulations, along with cell biology and genetic approaches, to investigate plant retrograde transport in the model plant Arabidopsis thaliana , with the following major findings: (1) The CLEM, 3D ET, and immunogold transmission electron microscopy (TEM) analyses revealed distinct spherical vesicles (~3050 nm in diameter) labeled by retromer markers and VSR proteins in Arabidopsis root cells, with nascent vesicles budding from MVBs in Arabidopsis root cells

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: Classification and adaptive behavior prediction of children with autism spectrum disorder based upon multivariate data analysis of markers of oxidative stress and DNA methylation

Fluorescence was read at Ex/Em = 535/587nm on BioTek HT Synergy Plate Reader

[PubMed] [Google Scholar] 356.Morton R.A