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Fe 2+ assay Intracellular Fe 2+ levels were measured utilizing the FerroOrange fluorescent probe (DOJINDO, Japan), in accordance with the guidelines provided by the manufacturer

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The membranes were blocked with 5% BSA or 5% skim milk, according to the manufacturers instructions

A narrow CO2 reserve combined with a high loop gain state is thought to be the main mechanism (Hernandez & Patil, 2016)