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This experiment was conducted in strict accordance with the ARRIVE standards 2.0 (Animal Research: Reporting of In Vivo Experiments)

Complex I dysfunction results in substantially reduced ATP production, creating an energy deficit that directly compromises sperm motility and cellular function ( 3.4 Molecular pathway interaction networks The reproductive toxicity induced by EEDs results from complex crosstalk among oxidative stress responses, metabolic disruption, and immune system activation rather than isolated pathway effects

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