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More recently, manufacturers have added a final ultrafiltration or depth filtration step to remove prions, which are infectious proteins that cause diseases like mad cow disease

In the case of bioluminescence resonance energy transfer (BRET), a donor such as the enzyme Renilla luciferase is used

This approach minimizes the intensity of initial side effects like water retention and flushing

After being washed in PBS, the slides were stained with hematoxylin and eosin, mounted with Canada balsam (03984, Sigma-Aldrich), and observed with an epifluorescence microscope (IX71, Olympus, Tokyo, Japan) equipped with a digital imaging system (DP71, Olympus)