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Large molecules wont fit into as many pores, and so their effective path length through the column will be shorter (Figure 5)

After washing with PBS containing 0.05% Tween20, the cells were treated with Alexa Fluor 555-conjugated anti-mouse IgG (Cell Signaling Technology)

In some cell types reduced ATP levels have been found to promote oxidative stress 50

Keep in mind that the body generally stores B12 in the liver for future use

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