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Because the LFCN is purely sensory, any other neurologic abnormalities on exam such as abnormalities in reflexes, strength, gait, or sensation outside the boundaries of the LFCN, indicate a process beyond just the LFCN

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5-EU click reaction was performed in buffer containing 100 mM Tris-HCl (pH 8.5), 2 mM CuSO 4 , 100 mM sodium ascorbate, and 5 M Alexa Fluor 488 Azide (Thermo Fisher Scientific, A10266) for 30 min in the dark followed by two washes with 1% (w/v) BSA in PBS

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Follow the prescribed dosage and duration for best results