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Behavioral testing was followed by biochemical analyses on colon, faces, serum, cerebellum, and prefrontal cortex, assessing tryptophan metabolites, serotonin (5-HT) pathway intermediates, neurotransmitters (GABA, glutamate, dopamine, acetylcholine) via UPLC, UPLC-TQD, and ELISA, alongside gut microbiota profiling through 16S r RNA sequencing

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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TLR2

Wang C, Zhang J, Wu H, Li ZM, Ye Q (2015) Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production