glutathione agarose column Sepharose 4B, Cytiva, affinity resin, pack of 10 mL optimized to yield up to 1mg of soluble GST tagged protein Thermo Scientific™ Pierce™ Glutathione Agarose

population frequency) predicted to bind p 7, hapten-p 7 and/or favor lysine at the C-terminal anchor position, identifying seven alleles including A*03 (Supplementary Fig

Mistake 3: Using sterile water instead of bacteriostatic water Sterile water is fine for single-use applications

This dimeric form of Prx2 is non-functional, but the disulfide bridge between the subunits can be broken by Trx, regenerating Prx2, and completing the catalytic cycle [73]

After ultracentrifugation at 200,000 g for 20 min at 4 C the resulting supernatants were used in the assays

is the rate at which alcohol is eliminated, averaging around 0.15 g/L/hr