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doi: 10.1016/j.intimp.2023.110503 102 ZhaoC

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Peptide separation was subsequently performed at 50 C and a flow rate of 300 nl min 1 on a 50 m 25 cm separation column (Acclaim PepMap RSLC, Dionex), applying a gradient ranging from 2.4 to 32.0% acetonitrile over the course of 90 min