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CE IVD one-step RT-qPCR kit detection of viral genes S and RdRP multiplex assay targeting the South African and Brazilian variant (mutation E484K FAM channel), the British variant (mutation A570D HEX channel), the viral RdRP gene (ROX channel) and the Exogenous Positive Control (Cy5 channel) E484K is a key so-called escape mutation that causes reduced binding of neutralizing antibodies and is found in variant B.1.351 (501Y.V2) referred to as the South African and P.1 (501Y.V3) referred to as the Brazilian variant A570D mutation is located exclusively in line B.1.1.7 (20I / 501Y.V1) referred to as the British variant

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In: Bergmayer HU (ed) Methods of enzymatic analysis Verlag Chemie

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