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Samples, standards and controls were added with internal standards working solution and tris (2-carboxyehtyl) phosphine hydrochloride solution (Merck KGaA, Darmstadt, Germany), or replace by Milli-Q water for cystine measurements, and vortexed at 800rpm for 15 min at room temperature

3g, FITC or Cy3 fluorescence of BEAGA/ApoE-PDHD pre-treated with saline (control) or 1 U mL 1 GGT was only detected on the cell sphere surface, suggesting that the nanoprobe could be endocytosed by a small number of peripheral cells, whereas BEAGA/ApoE-PDHD nanoprobe pre-incubated with 5 or 10 U mL 1 GGT penetrated deeply into the cell sphere

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Proc Natl Acad Sci USA 106:44024407 Wang K, Zhang S, Weber J, Baxter D, Galas DJ (2010) Export of microRNAs and microRNA-protective protein by mammalian cells