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For example, in the study of Oria et al., the authors studied the ligands spacing coupled to the stiffness of the substrate, in this case a simple 2D protein patterning hydrogels allowing to control the Young modulus and the disposition of the ligands (Oria et al., 2017)

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Hypothalamic brain-derived neurotrophic factor regulates glucagon secretion mediated by pancreatic efferent nerves