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Metabolite assays in cobalamin and folate deficiency

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The suspension was centrifuged at 15,000 g for 20 min at 4C, and the resulting supernatant was used directly as an enzyme source ( SOD (EC 1.15.1.1) activity was determined based on the capacity of the enzyme to inhibit the photoreduction of nitroblue tetrazolium (NBT) ( CAT (EC 1.11.1.6) activity was assayed, according to the method of 1 cm 1 ) as a result of H 2 O 2 degradation