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To account for differences in library size, gene-level expression data were normalized using the Trimmed Mean of M-values (TMM) method implemented in edgeR 138

Its a critical decision based on specific research objectives

Sethi: Skin Lightening Medically Reviewed by: Dr

The Ulp1-treated samples were incubated with Ni-NTA resin to remove the His14-SUMO tag, and the unbound proteins were concentrated and loaded onto a Superdex 200 Increase column equilibrated with the SEC buffer