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Methemoglobinemia is an immediate life-threatening condition to the mother and baby, whereas the risks of IV methylene blue administration are poorly defined and largely theoretical

J Biosci

For example, lower serotonin levels may affect concentrations of norepinephrine (noradrenaline) to lower levels which in turn may trigger depression (Balmus et al

Three-micron sections were prepared and mounted on slides, followed by deparaffinization and immunofluorescent staining according to a standard protocol as follows: For deparaffinization, the paraffin-embedded sections were placed at 60 C for at least 30 min, incubated twice in xylene (5 minutes each), and then transferred into 100% ethanol (2 minutes), 100% ethanol (2 minutes), 90% ethanol (1 minute), 80% ethanol (1 minute), and 70% ethanol (1 minute) at room temperature

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