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Primary ESCs were seeded into 12-well plates and cultured until they reached 6080% confluency, followed by transfection with 20 pmol STAT6 siRNAs per well using the Lipofectamine RNAiMAX reagent (Invitrogen, California, USA) in Opti-MEM (Gibco, New York, USA) according to the manufacturers instructions

and Grant ME 1567/9-1 to A.J.M.)

The longer time to onset of immune-related adverse events in our study underscores the importance of ongoing monitoring of patients during checkpoint inhibitor therapy, because such adverse events can occur at any time, and we found no patient factors that can help to predict their occurrence

A 2006 systematic review and meta-analysis concluded that such supplementation was associated with a 22% lower risk of cardiovascular defects in case-control studies and a 39% lower risk in cohort studies and randomized controlled trials (53)