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Npj Science of Food, 8 (1), Article 53

Kisselev, Pyotr, et al

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The sample preparation protocol is based on the simultaneous reduction with TCEP of disulfide bonds and derivatization of thiol groups with BCPB and the deproteinization of the mixture with PCA

Unlike the other two lipid modifications, S-palmitoylation achieves modification through unstable thioester bonds, a property that makes it the only reversible modification of protein lipidation (33)