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After 34 hours at 37C, the cells were treated with test reagents (growth factors compounds or antibodies), placed into the IncuCyte FLR for imaging, and allowed to form networks over the course of the 4 day experiment

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If there is any gap in the cold chain, assess how long the vial was at room temperature and whether that exceeds the allowable window

Choose models and readouts that align with each components preclinical literature mechanism