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248 In addition, IgG can promote the senescence phenotype of macrophages and microglia, release inflammatory factors such as IL-6 and TNF-, form a systemic chronic inflammatory environment, and accelerate multiorgan aging

For lipid reporter experiments, HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency), incubated for 48 h and imaged by confocal microscopy for membrane localized GFP

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This review summarizes the mechanisms by which MPs exposure induces gut microbiota dysbiosis and skin damage from an integrated gutskin axis perspective, highlighting their interplays relevance