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Description

The Detection of Percentage of Propidium Iodide (PI)-Positive Cell in the Whole Sciatic Nerve Hoechst 33,342 (Cat # H3570, ThermoFisher Scientific) and PI (Cat # P1304MP, ThermoFisher Scientific) were used to calculate the cell death percentage

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Two common tags (SUMO and FLAG) are cleaved by specific proteases without requiring the addition of an independent cleavage recognition site

DZ (SML0305), adenine (A2786), 1 M HCl (H9892), MTA (260585), DMSO (D8418), SAM (A7007), GSK591 (SML1751) and EPZ015666 (SML1421) were purchased from Sigma-Aldrich