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To assess phosphorylation in vitro, substrates GST-G6PD WT, GST-2YF or GST-2YA (1.5 g) was incubated with recombinant SRC (0.5 g) for 30 min at 37 C in a 50 l kinase reaction system (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 100 mM DTT, 10 mM MgCl 2 ) with 0.3 mM ATP

Chioccioli, M

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ADH2 is used by the yeast to convert ethanol back into acetaldehyde, and it is expressed only when sugar concentration is low

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