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The activated oocytes were then cultured in bicarbonate-buffered porcine zygote medium-5 (PZM-5) ( in vitro Culture (IVC) medium] and 7.5 mg/mL cytochalasin B for 3 h to suppress the extrusion of the pseudo-second-polar body

At that time, interest in KD for the treatment of this disease decreased and did not come to peoples attention again until the 1990s (2, 3), when its therapeutic effects were gradually found to be effective in a wide range of diseases and more therapeutic potentials of KD were proposed

It does this by improving some of the neurobiological disturbances typically caused by dietary restrictions

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Schug TT, Berry DC, Shaw NS, Travis SN, Noy N