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As a general guideline: 1-7 days at 4C, 1-4 weeks at -20C, and months to years at -80C

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For instance, it may affect the activity of enzymes such as hormone-sensitive lipase (HSL), which is one of the key enzymes in the axunge breakdown process and can catalyze the hydrolysis of triglycerides into diglycerol esters and free fatty acids

This concept forms the basis for various dietary restrictions and special diets, such as caloric restriction (CR), fasting or fasting-mimicking diet (FMD), ketogenic diet (KD), high-fat diet (HFD), or amino acid-defined diet

These trigger points can restrict motion, cause referred pain, and often appear in the neck, shoulders, or back