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Preosteoblasts produce large amounts of type I collagen as a major component of their surrounding matrix

Membranes were blocked with 5% BSA/TBST (1 hour), incubated overnight (4 C) with primary antibody against gp91phox (Santa Cruz, sc-130543, 1:1000), p47phox (Santa Cruz, sc-17845, 1:500), P-p47phox (pSer345) (Sigma, SAB4504721, 1:500), p40phox (Santa Cruz, sc-48388, 1:500), SOD-2 (Santa Cruz, sc-133134, 1:1000), HO-1 (Santa Cruz, sc-136960, 1:1000) followed by HRP-conjugated secondary antibody (Cell Signaling Technology, 7074, 7076)

E.B.T.: R01DK104998 (NIH)

This was followed by the quick addition of 1.250 mL of d-PLL (200 mM) as a coating ligand and 0.39 mL of ascorbic acid (0.1 M) as a reducing agent

Based on these pathological findings, the potential of antioxidants for the treatment of hearing loss has been demonstrated in several animal studies